Proteomics & Metabolomics Shared Resource

Overview of Services

The Proteomics and Metabolomics Shared Resource (PMSR) provides broad mass spectrometry services to academic and industry investigators.
The facility is affiliated with Atrium Health Wake Forest Baptist Comprehensive Cancer Center.
The PMSR’s mission is to provide access to advanced mass spectrometry techniques for the identification and quantitation of proteins and metabolites in biological samples.
In addition to the fee-for-service analyses, the PMSR offers options to accommodate various needs such as training, walk-up-and-use, and project-based collaborations.

Check our website: Proteomics and Metabolomics Shared Resource (PMSR)

Major Equipment and Technologies

Proteomics Service:

• Thermo Orbitrap Eclipse Tribrid mass spectrometer with Thermo Vanquish Neo and Evosep Eno nano-UHPLC systems
• Thermo Orbitrap Astral Zoom mass spectrometer with Evosep Eno nano-UHPLC system
• SCIEX ZenoTOF 8600 mass spectrometer with Waters Acquity M-Class UHPLC system
• Advion Triversa Nanomate Chip-ESI source with LESA capability
• Thermo AccelerOme Automated Sample Preparation Platform
• Software: Thermo Proteome Discoverer 3.1 with CHIMERYS; Biognosys Spectronaut 20; Bioinformatics Solutions PEAKS Studio 13.1; Thermo BioPharma Finder 4.1; Qiagen Ingenuity Pathway Analysis; Open source tools (e.g., Skyline, MaxQuant, DIA-NN) 

Metabolomics Service:

• SCIEX ZenoTOF 8600 mass spectrometer with Waters Acquity M-Class UHPLC system
• SCIEX Triple Quad 7500 mass spectrometer with Shimadzu UHPLC system
• Thermo Q Exactive HF mass spectrometer with Vanquish Horizon UHPLC system
• Shimadzu LCMS-8050 mass spectrometer with Shimadzu HPLC system
• Thermo GC Exploris 240 GS-MS/MS system (coming soon)
• Software: Thermo Compound Discoverer 3.4; Thermo LipidSearch 5.1; IROA Technologies MSMLS; SCIEX Molecular Profiler 1.3; SCIEX LipidView 1.2; Open source tools (e.g., MS-DIAL, XCMS Online)

 Leadership

Jingyun Lee, PhD, Director
Assistant Professor, Internal Medicine, Section on Molecular Medicine
Jingyun.Lee@advocatehealth.org
Hanes Building 2008 (Tel.336-713-4194) / 1 Medical Center Blvd, Winston-Salem, NC 27157 

Cristina M. Furdui, PhD, Scientific Advisor
Professor, Internal Medicine, Section on Molecular Medicine
Cristina.Furdui@advocatehealth.org
Nutrition Building C214A / 1 Medical Center Blvd, Winston-Salem, NC 27157

Staffs

Thais deAlmeida Pedrete, PhD, Staff Scientist Associate
Thais.Dealmeidapedrete@Advocatehealth.org
Hanes Building 2027 (Tel.336-713-1809) / 1 Medical Center Blvd, Winston-Salem, NC 27157

Ethan Underwood, MS, Lab Technician III
Ethan.Underwood@Advocatehealth.org
Hanes Building 2029A (Tel.336-716-2345) / 1 Medical Center Blvd, Winston-Salem, NC 27157

Hadi Pourhadi, PhD, Research Assistant
Hadi.Pourhadi@Advocatehealth.org
Hanes Building 2029A (Tel.336-716-2345) / 1 Medical Center Blvd, Winston-Salem, NC 27157 

Maria (Malu) L. Carneiro Buchele, PhD, Research Fellow
Maria.Carneirobuchele@advocatehealth.org
Nutrition Building 239 / 1 Medical Center Blvd, Winston-Salem, NC 27157  

Citation Resources

Cite the P30 CCSG
The authors wish to acknowledge the support of the Atrium Health Wake Forest Baptist Proteomics and Metabolics Shared Resource, supported by the National Cancer Institute’s Cancer Center Support Grant award number P30CA012197. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

NIHMS Guidelines for Authors
Authors should also make sure that all publications are processed by the National Institutes of Health Manuscript Submission (NIHMS) to obtain a PubMed Central reference number (PMCID), per NIH guidelines.

External Funding Application “Facility” Description
Review the facility description of this Shared Resource on pages 2-5 of the  AHWFCCC boilerplate. 

Hours of Operation

Monday - Friday 

9AM - 5PM

Contacts

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