Cell Engineering Shared Resource

Overview

Overview

The mission of the Cell Engineering Shared Resource (CESR) is to serve the scientific needs of Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) researchers. The CESR serves an essential function by providing investigators with considerable expertise in cell culture/cell line engineering/viral vector propagation, and the tools to analyze cell growth/death in vitro and in vivo under a wide variety of experimental conditions. The CESR is capable of designing, implementing, and optimizing new cell culture/transduction procedures on behalf of investigators, and can conduct short-term experiments efficiently in a cost-effective manner. The CESR supports the research community at Atrium Health Wake Forest Baptist campuses, allowing investigators to make the most of their time and research dollars. The CESR also offers an on-site stockroom for tissue culture reagents and custom procurement services with substantial discounts from multiple vendors.

Citation Resources

Cite the P30 CCSG

The authors wish to acknowledge the support of the Atrium Health Wake Forest Baptist Cell Entineering Shared Resource, supported by the National Cancer Institute’s Cancer Center Support Grant award number P30CA012197. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

NIHMS Guidelines for Authors

Authors should also make sure that all publications are processed by the National Institutes of Health Manuscript Submission (NIHMS) to obtain a PubMed Central reference number (PMCID), per NIH guidelines.

Research Project Support (Contact Ravi.Singh@advocatehealth.org or Jolyn.Turner@Advocatehealth.org)

1. Customized assistance for cell culture/cell line engineering techniques including gene editing (CRISPR)/ and viral/plasmid vector transduction/ cell viability/growth analysis
2. CRISPR design and optimization (in partnership with Sigma-Aldrich’s CRISPR Core Partnership Program)
3. Consultation and customized assistance to aid in design, implementation and optimization and trouble-shooting cell of new cell culture procedures
4. Production and concentration of replication-incompetent viruses including: lentivirus (ecotrophic and amphotrophic); retrovirus (we have a variety of CESR prepared lentivirus stocks available for purchase)
5. Plasmid expansion (tranformation; mini/midi/maxiprep; endotoxin free DNA purification)
6. Quality control and detailed records for each research project

Products and Services (contact Yelena.Karpova@Advocatehealth.org):

Visit our on-line service page for the following at http://ccc.wfubmc.edu/corelab/serviceorder.aspx:

1. On-site stockroom and distribution center with discounted pricing for cell culture media (including FBS), reagents and plastics (Fisher, ISCBioexpress); same day pick-up available
2. Daily ordering and significant discounts for non-stockroom products and services from external vendors including BioRad, eBioscience, Gemini, Lonza, LifeTech, PerkinElmer, Qiagen, Roche, Sigma
3. Free shipping/packaging/handling (including dry ice shipments) for all orders with distribution to all campuses daily
4. Ordering of primers from Invitrogen; orders placed daily

Visit our CESR iLab Service Requests page for:

1. Lentiviral particles prepared in the CESR (100X concentrated VSV-G pseudotyped lentiviral particles)
2. Custom media and reagent preparation under strict quality control
3. Establishment of cells from a cell line repository (over 100 cancer cell lines)
4. Cryopreservation and storage of tissue culture cells (liquid nitrogen)
5. Mycoplasma testing and control

Equipment we support:

IVIS Lumina Series III

• Non-invasive (fluorescence/luminescence) imaging of cells in rodents.

X-Ray Film Processor

• Gray 5070

Biospherix Xvivo X3 Hypoxia Hood & Culture Comb (coming soon)

• Growth of cells in low oxygen (hypoxic) conditions

Leadership

Ravi Singh, Ph.D

Department of Cancer Biology

Ravi.Singh@advocatehealth.org

Staff

Yelena Karpova: Yelena.Karpova@Advocatehealth.org

Jane Strupe: Jane.Strupe@Advocatehealth.org

JoLyn Turner, Ph.D.: Jolyn.Turner@Advocatehealth.org

Location and hours of operation

Links and Resources

1.  https://school.wakehealth.edu/research/institutes-and-centers/comprehensive-cancer-center/shared-resources-and-cores/cell-engineering

Citation Resources

Cite the P30 CCSG

The authors wish to acknowledge the support of the Wake Forest Baptist Cell Engineering Shared Resource, supported by the National Cancer Institute’s Cancer Center Support Grant award number P30CA012197. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

NIHMS Guidelines for Authors

Authors should also make sure that all publications are processed by the National Institutes of Health Manuscript Submission (NIHMS) to obtain a PubMed Central reference number (PMCID), per NIH guidelines.

External Funding Application “Facility” Description

Review the facility description of this Shared Resource on pages 2-5 of the  AHWFCCC boilerplate. 

Contacts